Sepax Nanofilm SEC Columns

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Sepax Nanofilm SEC Columns Trade Information

Minimum Order Quantity
10 boxes Boxes

FOB Port
MUMBAI

Payment Terms
Cash Against Delivery (CAD), Cash on Delivery (COD), Cheque, Delivery Point (DP), Letter of Credit (L/C), Western Union, Letter of Credit at Sight (Sight L/C), Telegraphic Transfer (T/T), Days after Acceptance (DA), Cash in Advance (CID), Cash Advance (CA)

Supply Ability
500 Boxes Per Day

Delivery Time
4 Week

Sample Available
Yes

Sample Policy
Free samples are available

Main Export Market(s)
Australia, North America, South America, Eastern Europe, Western Europe, Middle East, Africa, Asia, Central America

Main Domestic Market
All India

About Sepax Nanofilm SEC Columns

Nanofilm Technical Specifications

Phase	Nanofilm SEC-150	Nanofilm SEC-250	Nanofilm SEC-500
Material	Nanometer, hydrophilic film bonded silica
Particle size	5 m
Pore size	~ 150	~ 250	~ 450
Protein MW range (native)	200 - 750,000	1,500 - 1,250,000	15,000 - 2,500,000
pH stability	2 - 8.5 (pH 9.0 can be tolerated temporarily)
Back pressure (psi for a 4.6 x 300mm)	~ 700	~ 700	~ 750
Maximum back pressure (psi)	~ 3,500	~3,500	~ 3,000
Salt concentration range	20 mM - 2.0 M
Maximum temperature (°C)	~ 80

For biological separations, nonspecific interactions with the packing materials are the major contribution for low separation efficiency, such as peak tailing, as well as low recovery. The unique surface chemistry of Nanofilm SEC packings generates uniform polymer chains covalently bonded on the silica. Such a uniform stationary phase is neutral and hydrophilic, which has negligible nonspecific interactions with biological molecules, especially proteins. This special coating allows Nanofilm SEC columns to achieve high selectivity and high efficiency separations. For example, uracil, as a test compound could reach as high efficiency as 90,000 plates per meter, as shown in Table below.

Table 1. Column Efficiencies (Measured using uracil in 0.1 M phosphate buffer, pH 7.0)