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Sepax Nanofilm SEC Columns

Sepax Nanofilm SEC Columns

Product Details:

100000.00 - 200000.00 INR/Box
X

Sepax Nanofilm SEC Columns Price And Quantity

  • 10 boxes Box
  • 100000.00 - 200000.00 INR/Box

Sepax Nanofilm SEC Columns Trade Information

  • MUMBAI
  • Cash Against Delivery (CAD) Cash on Delivery (COD) Cheque Delivery Point (DP) Letter of Credit (L/C) Western Union Letter of Credit at Sight (Sight L/C) Telegraphic Transfer (T/T) Days after Acceptance (DA) Cash in Advance (CID) Cash Advance (CA)
  • 500 Box Per Day
  • 4 Week
  • Yes
  • Free samples are available
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  • All India

Product Description

Nanofilm Technical Specifications

Phase

Nanofilm SEC-150

Nanofilm SEC-250

Nanofilm SEC-500

Material

Nanometer, hydrophilic film bonded silica

Particle size

5 m

Pore size

~ 150

~ 250

~ 450

Protein MW range (native)

200 - 750,000

1,500 - 1,250,000

15,000 - 2,500,000

pH stability

2 - 8.5 (pH 9.0 can be tolerated temporarily)

Back pressure (psi for a 4.6 x 300mm)

~ 700

~ 700

~ 750

Maximum back pressure (psi)

~ 3,500

~3,500

~ 3,000

Salt concentration range

20 mM - 2.0 M

Maximum temperature (°C)

~ 80

For biological separations, nonspecific interactions with the packing materials are the major contribution for low separation efficiency, such as peak tailing, as well as low recovery. The unique surface chemistry of Nanofilm SEC packings generates uniform polymer chains covalently bonded on the silica. Such a uniform stationary phase is neutral and hydrophilic, which has negligible nonspecific interactions with biological molecules, especially proteins. This special coating allows Nanofilm SEC columns to achieve high selectivity and high efficiency separations. For example, uracil, as a test compound could reach as high efficiency as 90,000 plates per meter, as shown in Table below.

Table 1. Column Efficiencies (Measured using uracil in 0.1 M phosphate buffer, pH 7.0)

Nanofilm SEC-150

>90,000 plates/meter

Nanofilm SEC-250

>90,000 plates/meter

Nanofilm SEC-500

>85,000 plates/meter

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