Sepax Polar Mc Ni-mc60 Imac Resin
Sepax Polar Mc Ni-mc60 Imac Resins are functional as the metal affinity chromatography resins, suited for the effective purification of recombinant proteins. These are highly demanded for the biotech industry and are appreciable for their highly hydrophilic nature. Sepax Polar Mc Ni-mc60 Imac Resins are suitable for pilot, R&D, and industrial scale runs. These are high-purity resins of optimum quality and enhanced utility. They are suited for several industrial tasks. These are the metal affinity chromatography resins suited for the purification of recombinant proteins.
Resin Type | Polar MC30-CA Excel, Polar MC30-Ni Excel |
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Packing | 50% (v/v) slurry in 20% ethanol |
Matrix | Hydrophilic polymethacrylate |
Particle Size | 20 ~ 45 µm (mean 30 µm) |
Pore Structure | 800 … |
pH Stability | 1-13 for base resin, Polar MC-CA Excel |
Loading Capacity | Polar MC30-CA Excel -> 120 µeq. carboxylic group /mL |
HIS-Protein Loading | Polar MC30-Ni Excel, ~20 mg/mL |
Operating Temperature | Up to 40 °C |
Operating Pressure | Up to 60 bar |
Mobile Phase Compatibility | Aqueous solutions, a mixture of water and acetonitrile, acetone, or methanol. Typical buffers: phosphate, Tris, and acetate, also compatible with Guanidine HCl 6 M, Urea 8 M, EDTA 0.1 M, DTT 10 mM |
Linear Flow Rate | Up to 1800 cm/hour |
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