Polar MC-IMAC Excel resins are metal affinity chromatography resins for the purification of recombinant proteins with a histidine (His) tag, which are widely used in the biotech industry. The resins are composed of hydrophilic polymethacrylate beads with high physical and chemical stability. The resins have a particle size of 30 µm or 60 µm with a pore size of 800.
Resin Type | Polar MC30-CA Excel, Polar MC30-Ni Excel |
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Packing | 50% (v/v) slurry in 20% ethanol |
Matrix | Hydrophilic polymethacrylate |
Particle Size | 20 ~ 45 µm (mean 30 µm) |
Pore Structure | 800 |
pH Stability | 1-13 for base resin, Polar MC-CA Excel |
Loading Capacity | Polar MC30-CA Excel -> 120 µeq. carboxylic group /mL |
HIS-Protein Loading | Polar MC30-Ni Excel, ~20 mg/mL |
Operating Temperature | Up to 40 °C |
Operating Pressure | Up to 60 bar |
Mobile Phase Compatibility | Aqueous solutions, a mixture of water and acetonitrile, acetone, or methanol. Typical buffers: phosphate, Tris, and acetate, also compatible with Guanidine HCl 6 M, Urea 8 M, EDTA 0.1 M, DTT 10 mM |
Linear Flow Rate | Up to 1800 cm/hour |
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